Cashless Transactions - Mark of the Beast

CRISPR-Based Therapeutics Blaze an In Vivo Path to the Clinic. “Guide RNAs have become mainstays of CRISPR genome ‘EDITING’ technology.” More ambitious CRISPR-based in vivo therapies are now in development which allow GENES to be EDITED INSIDE of the PATIENT. 2019-2024 = ‘5’ years. Genetic Engineering and Biotechnology News . A ‘Strongly Encouraged’ Read. MODERNA ‘MODify RNA.’

Ezekiel 33:6

But if the watchman see the sword come, and blow not the trumpet, and the people be not warned; if the sword come, and take any person from among them, he is taken away in his iniquity; but his blood will I require at the watchman’s hand.

Ezekiel 33:3

If when he seeth the sword come upon the land, he blow the trumpetand warn the people;

Ezekiel 33:4

Then whosoever heareth the sound of the trumpet, and taketh not warning; if the sword come, and take him away, his blood shall be upon his own head.

Ezekiel 33:5

He heard the sound of the trumpet, and took not warning; his blood shall be upon him. But he that taketh warning shall deliver his soul

The Coming Biometric Vaccination Mark of the Beast System:

(Five (5); Digital ID, Vaccination Passport, Biometric Infrared Scanning, Vaccine ‘Mark’ or ‘code/number’ of Approval or Authorization, 5G Technology/Network.)

The ‘coming’ mark will most likely involve a ‘subdermal’ (non-hypodermic needle) application of the next wave of vaccinations, that will include a form of ‘digital data memory’ indicating date and time of vaccination. This ‘mark’ of vaccination will involve a sub-dermal outline or image representing a ‘branding’ that can be scanned and revealed by infrared light at a retail checkout point scanner. Just as many people have a physical vaccination ‘mark’ on their arm from getting shots in the 1960s, the coming vaccination mark will be subtle and reside ‘inside’ the human body affecting and indicating a genomic change in the recipient. This is an abomination viewed by God and causes the recipient to lose ‘Imager Status before God’ and results in eternal damnation. The vaccination ‘mark’ has to be easily accessible by scanners and retail checkout points, hence the recipient will have the option of receiving the vaccination ‘patch’ either on their hand or forehead for ‘scannability.’ The patch or medium itself ‘dissolves’ on the exterior of the skin, as the vaccination and information is administered or delivered under the skin. Some technology is currently under development that will possibly allow for the recipient’s health status and response to the vaccine to be ‘scanned’ and ‘verified’ or ‘validated’ at retail or social checkpoints. The purpose of which is to identify whether or not the vaccine has actually worked (and is working) on the recipient’s body / immune system / genetics / RNA / DNA, in addition to informing authorities as to date, time, dosage and manufacturer of said ‘medicine.’

https://www.prnewswire.com/news-releases/pope-francis-joins-the-ad-council-and-covid-collaboratives-its-up-to-you-campaign-to-inspire-confidence-in-the-covid-19-vaccines-301357521.html

https://www.freep.com/story/news/health/2021/08/18/pope-francis-covid-19-vaccination-psa-coronavirus/8171706002/

https://www.euronews.com/2021/08/18/pope-francis-backs-covid-19-vaccination-as-an-act-of-love-in-new-video?utm_source=newsletter&utm_medium=en&utm_content=pope-francis-backs-covid-19-vaccination-as-an-act-of-love-in-new-video&_ope=eyJndWlkIjoiODE3MTBhNWMxNTEzNmY3YWNkM2ZjMjBlOGJkZGMwMjQifQ%3D%3D

https://english.alarabiya.net/coronavirus/2021/08/18/Pope-Francis-appears-in-video-promoting-COVID-19-vaccination

False Prophet (The WORLD’S Greatest Social ‘Influencer’). ‘Go ahead, get the jab, it is good for you, it is good for humanity, it is the ‘moral’ thing to do.’

Revelation 13:16-17 And he [false prophet, pope, pontiff] causeth ALL, both small and great, rich and poor, free and bond, to receive a [‘subdermal, infrared, vaccination, smart’] mark IN their right hand, or IN their foreheads: And that no man might buy or sell, save he that had the [‘subdermal, infrared, vaccination, smart’] mark, or the name of the beast, or the number of his name.

Revelation 19:20 

And the beast was taken, and with him the false prophet that wrought miracles before him, with which he [false prophet, pope, pontiff] DECEIVED THEM that had received the [‘subdermal, infrared, vaccination, smart’] mark of the beast, and them that worshipped his image. These both were cast alive into a lake of fire burning with brimstone.

Revelation 14:11

And the smoke of their torment ascendeth up for ever and ever: and they have no rest day nor night, who worship the beast and his image, and whosoever receiveth the mark of his name.

Revelation 20:4

And I saw thrones, and they sat upon them, and judgment was given unto them: and I saw the souls of them that were beheaded for the witness of Jesus, and for the word of God, and which had NOT worshipped the beast, neither his image, neither had received his mark upon their foreheads, or in their hands; and they lived and reigned with Christ a thousand years.

5G + Biometric Facial / Hand Scanning + Digital / Cashless Transactions + Mark of Approval / Authorization = Tomorrow’s Economy (2020-2027). Why 2020? Because these ‘things’ have already occurred (past tense) elsewhere (China, India, Asia and a few countries in Europe) in the world in 2020, even though they have not yet occurred in the ‘United’ States.

Biometric scanning used for migrant/border control, secure identity verification and approval, government benefits, domestic and international travel security, economic inclusion of everyone, buying/selling transactions and fund transfers, instantaneous speed of transaction and convenience, VAT/taxation collection, mobility, social credit score and ranking, population movement and tracking, monitoring political and religious ‘extremism’ (terrorists). ‘Mark, number or name’ for verification and approval.

‘Any foreign material(s) should NOT be injected into the human body that causes any kind of change in genetic makeup or structure of a human being. This could be five (5) genetic ‘manipulation’, ‘enhancement’, ‘cutting’, ‘modification’, or ‘alteration.’

Revelation 16:2

And the first went, and poured out his vial upon the earth; and there fell a noisome and grievous sore upon the men which had the mark of the beast, and upon them which worshipped his image. [Symptom or result from having ‘received’ the mark, a result from having changed or damaged a person’s RNA, DNA, Immune System or Genetics].

Both American vaccines use messenger RNA (mRNA) to combat the virus. That is an advanced genetic therapy that uses the virus’ own genetic material against it. Kendrick, however, warns that the technology isreally new and untested, meaning no one really knows how it could affect human health, since it literally hijacks the cellular reproduction mechanism. “We really do not know what these things might end up doing after a prolonged period of time.”

“The plague year of 2020 will be remembered as the time when traditional vaccines were supplanted by something fundamentally new: genetic vaccines, which deliver a gene or piece of genetic code into human cells. The genetic instructions then cause the cells to produce, on their own, safe components of the target virus in order to stimulate the patient’s immune system.”

‘The MARK’ may not be here just yet, but it will be shortly … in terms of a year or two (2023). Verification that a ‘genetic manipulation’ has been administered and will be evident, either in the hand or in the forehead by infrared biometric scanning to enable travel, buying and selling. Do you still ‘want to take’ the jab even if it is not yet administered in your hand or in your forehead in some other manner? If you are curious, please read my other analyses on ‘why’ getting ‘the mark’ is such a bad (understatement) thing in the eyes of God. It is one thing to understand that people in the extremely near future should NOT GET THE MARK. It is another thing to UNDERSTAND WHY THEY SHOULD NOT GET A MARK. This is not a ‘conspiracy theory,’ but the Word of God. I have provided the requisite verses from the Bible, regarding God’s comments about ‘the mark’ of the coming world beast system.

NO ‘MICROCHIP’ NEEDED. Implantable Quantum Dot Microneedle Vaccine INFRARED Delivery System:

MIT researchers have now developed a novel way to record a patient’s vaccination history: storing the data in a pattern of dye, invisible to the naked eye, that is delivered under the skin at the same time as the vaccine. This technology could enable the rapid and anonymous detection of patient vaccination history. The researchers showed that their new dye, which consists of nanocrystals called quantum dots, can remain for at least five (5) years under the skin, where it emits [near-infrared light] that can be detected by a specially equipped smartphone. To create an “on-patient,” decentralized medical record, the researchers developed a new type of copper-based quantum dots, which emit light in the [near-infrared spectrum]. The dots are only about 4 nanometers in diameter, but they are encapsulated in biocompatible microparticles that form spheres about 20 microns in diameter. This encapsulation allows the dye to remain in place, under the skin, after being injected. The researchers designed their dye to be delivered by a microneedle ‘patch’ rather than a traditional syringe and needle. Such patches are now being developed to deliver vaccines for measles, rubella, and other diseases, and the researchers showed that their dye could be easily incorporated into these patches. The microneedles used in this study are made from a mixture of dissolvable sugar and a polymer called PVA, as well as the quantum-dot dye and the vaccine. When the patch is applied to the skin, the microneedles, which are 1.5 millimeters long, partially dissolve, releasing their payload within about two minutes.

By selectively loading microparticles into microneedles, the patches deliver a pattern in the skin that is invisible to the naked eye but can be scanned with a smartphone that has the [infrared] filter removed. The patch can be customized to imprint different patterns that correspond to the type of vaccine delivered. “It’s possible that this ‘invisible’ approach could create new possibilities for data storage, biosensing, and vaccine applications that could improve how medical care is provided, particularly in the developing world,” Langer says.

The quantum-dot patterns could be detected by smartphone cameras after up to five (5) years of simulated sun exposure. The researchers are also working on expanding the amount of data that can be encoded in a single pattern, allowing them to include information such as the date of vaccine administration and the lot number of the vaccine batch. “Storage, access, and control of medical records is an important topic with many possible approaches.” “This study presents a novel approach where the medical record is stored and controlled by the patient within the patient’s skin in a minimally invasive and elegant way.” The research was funded by the Bill and Melinda Gates Foundation and the Koch Institute Support (core) Grant from the National Cancer Institute.

APPLE FACE and PALM ‘INFRARED Photo Detectors’ biometrics planned in 2023 iPhones.

Face ID Infrared biometrics cameras are coming to Apple devices in 2023. A series of patent filings by Apple have hinted at the company’s work on under-display biometric cameras, with the latest published in February (2021).  Apple eyes next-generation biometric scanners for under-display Touch ID. ‘[Infrared photodetectors] could be used for biometric user identification and authentication in the palm or face.’ A new patent application by Apple is signaling the company’s emphasis on biometric ‘photodetector’ technology for under-display touch identification to be potentially deployed on its devices. The sensors include biometric sensors, depth sensors, and cameras. Apple’s listed uses of the photodetectors include security and health monitoring. Arrays of these photodetectors may be deployed to scan fingerprint, palm print, 3D face, or eye biometrics. The [photodetectors could be used for biometric user identification and authentication.] Photodetectors emit electromagnetic radiation against an object such as a finger, face, or stylus. Once reflected by the object, this radiation returns a backscatter which then passes back through the display to register the object’s information. The document also refers to the possibility of using optical, ultrasonic, thermal or other sensing technologies within the system. In addition to this, the photodetectors are also capable of obtaining ophthalmic scans, ECG, and pulse for health monitoring. Other uses of the scanners include palm scanning for social matching and social networking. Apple’s research and development of technology for monitoring health metrics, three patent applications describe [Infrared transceivers] for object recognition. The [infrared transceivers], which may be used in telephones include beam-steering technology and will likely be used for augmented object recognition via visible and [infrared light]. According to the filing, the device can sense objects in a variety of light conditions including visible light, UV, and [infrared spectrum] ranges.

Vaccine INFRARED ‘Micro-Needle SMART Patch’

“Nanocrystals called quantum dots, can remain for at least FIVE (5) years under the skin, forming an ‘image’, where they emit near-[infrared light] that can be detected by a specially equipped smartphone.” (MIT researchers FUNDED by the Bill & Melinda Gates Foundation).

‘Infrared’ Mark of the Beast, 2023 to 2028 = FIVE (5) YEARS.

FIVE (5) is the number denoting Satanic influence over the behavior of evil man’kind (666).

2023 ‘INFRARED’ SMART Phones

2023 ‘INFRARED’ VACCINE MICRO-NEEDLE SMART Patches

Come June, 2021 … 2023 is ONLY 18 (6+6+6) months in the future.

False Prophet

Revelation 13:16-17 And he causeth all, both small and great, rich and poor, free and bond, to [receive a mark IN their right hand, or IN their foreheads]:And that no man might buy or sell, save he that had the mark, or the name of the beast, or the number of his name.

My discernment is that;

2023 will mark the ‘roll-out’ of the ‘MARK’ of the BEAST.

2024 will mark the ‘mid-point’ of the 7 Year Tribulation, the Abomination of Desolation revealing the BEAST to be the ANTICHRIST, the Destruction of ‘Mystery Religion’, the beginning of worshipping the BEAST and his IMAGE, and the ‘BEHEADING’ of those who refuse the  Beast’s MARK, refuse to worship the BEAST or his IMAGE. Until 2024, Christians will be ‘protected’ under the MYSTERY RELIGION doctrine of (5) ‘inter-religious dialogue, brotherhood, human fraternity, coexistence and false religious / political peace.’ Upon the enactment of the Antichrist’s Abomination of Desolation and the abolishment of the Mystery Religion, is when BOTH the Jews will become persecuted and have to flee Jerusalem AND Christians will start to be BEHEADED because they will refuse to get the mark and refuse to worship the BEAST and his IMAGE. Beheadings will last during the FINAL 3.5 years of the Tribulation (Great Tribulation) and cease at the end of 2027. 2028 (8) will usher in the Jew’s REDEMPTION, sheep and goat nations’ judgement, gathering of the remaining ‘elect’ from the four corners of the earth and the beginning of Jesus Christ’s 1,000 Year Millennial Reign upon earth. The Second, Physical Coming of Christ to Earth (Great Day of the Lord and Armageddon) will likely take place near the very end of (fall, on/about November 1, 2027) 2027.

God will NOT let Christ’s Bride be Deceived by the Dragon, or his ‘MARK.’

True Church / Bride of Christ Spared from God’s Wrath:

 Romans 5:8-10. “But God commendeth his love toward us, in that, while we were yet sinners, Christ died for us. Much more then, being now justified by his blood, we shall be saved from wrath through him. For if, when we were enemies, we were reconciled to God by the death of his Son, much more, being reconciled, we shall be saved by his life.”

1 Thessalonians 1:10. And to wait for his Son from heaven, whom he raised from the dead, even Jesus, which delivered us from the wrath to come.

1 Thessalonians 5:9. For God hath not appointed us to wrath, but to obtain salvation by our Lord Jesus Christ,

Romans 8:35. Who shall separate us from the love of Christ? shall tribulation, or distress, or persecution, or famine, or nakedness, or peril, or sword?

Jeremiah 30:7. Alas! for that day is great, so that none is like it: it is even the time of Jacob’s trouble, but he shall be saved out of it.

Revelation 3:10 Because thou hast kept the word of my patience, I also will keep thee from the hour of temptation, which shall come upon all the world, to try them that dwell upon the earth.

The ‘mark’ comes shortly, during the tribulation, but the Bride is ‘not here.’ The ‘current’ vaccine(s) are NOT the mark, yet. But they are a ‘precursor’ (‘NORMALIZATION’) of the beast system process, getting people accustomed to getting ‘tagged’, ‘marked’ or ‘branded.’

CRISPR-Based Therapeutics Blaze an In Vivo Path to the Clinic. “Guide RNAs have become mainstays of CRISPR genome ‘EDITING’ technology.” More ambitious CRISPR-based in vivo therapies are now in development which allow GENES to be EDITED INSIDE of the PATIENT. 2019-2024 = ‘5’ years. Genetic Engineering and Biotechnology News .

Pioneering companies are showing that CRISPR systems can be tucked inside delivery vehicles and injected locally or systemically to bring about cures

By Ashleen Knutsen  September 1, 2021 Genetic Engineering and Biotechnology News

Therapeutic applications of genome editing were envisioned at least as early as the mid-1990s, when the first sequence-specific genome editing technologies emerged. Initially, such applications were considered distant prospects, but by 2012 (2012 + 10 ‘5+5’ years = 2022), they suddenly seemed near to hand. It was at that time that CRISPR technologies emerged.

‘As in the days of Noah, ALL FLESH HAS BECOME CORRUPTED, DEFILED OR UNLCEAN’ (GENETIC CORRUPTON / MANIPULATION)

Pastor J.D.F. begins about one minute into the the video:

CRISPR, which stands for clustered regularly interspaced short palindromic repeats, came to notice as a curious pattern in bacterial DNA. Attempts to understand CRISPR led to the discovery of bacterial immune systems. Even better, these systems were found to include RNA-programmable nucleases, that is, CRISPR-associated proteins (Cas proteins) that complex with guide RNAs to target specific DNA sequences.

Cas proteins and guide RNAs have become mainstays of CRISPR genome editing technology, and this technology has been used to realize ex vivo therapies. For example, CRISPR-modified T cells are being deployed in immunotherapies, and CRISPR-modified hematopoietic stem cells are showing promise against sickle-cell disease and β-thalassemia.

More ambitious CRISPR-based in vivo therapies are now in development. These therapies, which allow genes to be edited inside patients, would be impossible but for the development of improved CRISPR-based genome editing tools, such as the alternative or modified Cas proteins discussed in this article. When these tools are encapsulated by viral capsids or lipid nanoparticles (LNPs) [Please see my foreward regading MicroNeedle Vaccine ‘SMART’ Patches that use ‘nano-particles’ for the encapsulation of vaccines], they constitute therapeutics that may be administered locally, within specific tissues, or systemically throughout the entire body.

In vivo CRISPR therapy enters the clinic

In November 2020, the first systemically delivered CRISPR-Cas9 therapy entered clinical trials. This therapy, which is called NTLA-2001, is being developed by Intellia Therapeutics and Regeneron. NTLA-2001 consists of a lipid nanoparticle that is designed to target the liver, and that encapsulates a Cas9-enzyme-encoding messenger RNA and a guide RNA. Once expressed within liver cells, the Cas9 enzyme is directed by the guide RNA to the gene for transthyretin (TTR).

Through targeted knockdown of the gene for TTR, NTLA-2001 reduces the serum concentration of the protein. In this way, NTLA-2001 treats TTR amyloidosis, a rare genetic disease that is caused by the accumulation of misfolded TTR in tissues such as the nerves, heart, and kidneys. The therapy is administered just one time, through an intravenous injection (Night Watchman; ‘intravenous injections’ aka needles, will be replaced by ‘injection’ via Infrared Microneedle Vaccine SMART Patch that use nanoparticles and gene editing ‘vaccines’.)

In a Phase I clinical trial that included six patients, an 87% average reduction in serum TTR was observed for the patients who received the highest dose (Gillmore et al. N. Engl. J. Med. 2021; DOI: 10.1056/NEJMoa2107454). The reductions in serum TTR could be a durable effect, suggest the developers of NTLA-2001. They point to preclinical studies in which deep and long-lasting TTR reductions were observed in rodents and nonhuman primates. In fact, TTR levels remained low even when the drug was evaluated in a rodent model of accelerated liver regeneration.

“Because we’re changing the DNA, the genetic makeup of that cell, the expectation is that once you do that, that cell will never revert back,” says Laura Sepp-Lorenzino, PhD, executive vice president and chief scientific officer at Intellia Therapeutics. “These technologies in medicine will have a long-lasting, potentially curative effect in humans.” (Night Watchman; ‘LOST IMAGER STATUS’, AS IN THE DAYS OF NOAH, ALL FLESH HAS BECOME CORRUPTED – DEFILLED – UNCLEN, GENETIC DEFILEMENT OF THE HUMAN GENOME).

In the clinical study, researchers assessed NTLA-2001’s potential for deleterious off-target effects by performing genome-wide assays and targeted sequencing. The researchers identified seven candidate off-target sites, all of which were located in noncoding regions. For these sites, no detectable levels of off-target editing were found, even when primary cell cultures of human hepatocytes were treated with concentrations of NTLA-2001 that were up to three times higher than the 90% maximal effective concentration.

Alternatives to Cas9

Although the CRISPR-associated protein derived from the Streptococcus pyogenes nuclease, known as SpCas9 or Cas9, is the most widely used Cas protein, it is not the only option. Alternatives are being explored by several companies. For example, AsCas12a is being developed by Editas Medicine.

AsCas12a is derived from a gut bacterium called Acidaminococcus sp. Studies have demonstrated that this protein has fewer off-target effects than Cas9. However, it also has a lower editing efficiency, meaning fewer cells receive the desired edit.

Editas has been working to address this limitation. In collaboration with Integrated DNA Technologies (IDT), Editas has developed a more active version of AsCas12a that is called AsCas12a Ultra. Scientists representing the companies published a study (Zhang et al. Nat. Commun. 2021; 12: 3908) indicating that AsCas12a Ultra, like AsCas12a, has much better on-target specificity than Cas9. AsCas12a Ultra was also shown to be nearly 100% efficient at gene editing across all cell types and target sites tested, a capability that has never been seen with any other gene editing nuclease. Finally, AsCas12a Ultra was shown to be highly potent.

“A more potent nuclease means you need to add less of your CRISPR-Cas agent to achieve the same effect,” says John Zuris, PhD, associate director of editing technologies at Editas. “This is especially important for multiplexed editing, where you need to add many different CRISPR-Cas agents at the same time. We show that AsCas12a Ultra is, on average, over 40-fold more potent than wild-type AsCas12a.”

Editas has engineered an AsCas12a protein based on AsCas12a Ultra for cell therapy. Currently, the engineered protein is being evaluated for its ability to treat sickle-cell disease. The company is now enrolling patients for a Phase I/II study.

Another alternative to SpCas9 that the company is exploring is a nuclease derived from Staphylococcus aureus. This nuclease is called SaCas9, and it engages in less off-target activity than SpCas9. Another advantage offered by SaCas9 is its compactness, a quality that Editas is exploiting to develop a treatment for an inherited retinal disorder, Leber congenital amaurosis (LCA), that is caused by mutations in the CEP290 gene.

The treatment consists of an adeno-associated virus (AAV) vector that incorporates a plasmid that expresses SaCas9 and human CEP290-specific gRNAs. When EDIT-101 is administered via a subretinal injection, it delivers the gene editing machinery directly to photoreceptor cells.

“SaCas9 is smaller than SpCas9 and enables us to package all the CRISPR-Cas elements in a single AAV vector for delivery to photoreceptors,” Zuris asserts.

In vivo base editing

The familiar SpCas9 nuclease and the relatively new alternatives cited thus far in this article are not the only tools in the CRISPR gene editing toolbox. Other options are engineered enzymes that eschew nuclease activity and instead serve as base editors.

The first two base editors, a cytosine-to-thymine base editor (CBE) and an adenosine-to-guanosine base editor (ABE), appeared in 2016 and 2017, respectively. Both were developed in a laboratory led by David Liu, PhD, at Harvard University. Both consist of a catalytically impaired Cas9 fused to a base-swapping enzyme.

Because the base editors incorporate catalytically impaired Cas9, they don’t create a double-strand break in the DNA, and they don’t rely on the cell’s repair system to make the desired changes. Instead, the base editors cause single-strand breaks. At each break, a base editor will swap one base for another.

According to a recent study (Anzalone et al. Nat. Biotechnol. 2020; 38: 824–844) from Liu’s team, base editors could theoretically be used to correct approximately 30% of the known pathogenic point mutations listed in ClinVar, a public database developed by the U.S. National Institutes of Health.

An ABE base editor is the lead product candidate at Verve Therapeutics, a company that focuses on cardiovascular diseases. This base editor is called VERVE-101, and it is being used to inactivate the PCSK9 gene and reduce levels of low-density lipoprotein (LDL) cholesterol in blood.

Like NTLA-2001, Verve’s therapy uses LNPs (Liquid Nano-Particles) to deliver messenger RNA that, in this case, encodes for ABE as well as guide RNA to the liver. It is also designed as a one-time, ‘intravenous’ injection and, so far, has had very promising results.

In a recent study on nonhuman primates (Musunuru et al. Nature 2021; 593: 429–434), researchers at Verve Therapeutics found their therapy maintained an 89% reduction in PCSK9 expression and a 59% reduction of LDL for the eight months of the study. As with the NTLA-2001 results, this result suggests that even as the liver regenerates, the edits to the DNA remain. In addition, the researchers found no evidence of edits at the top 141 sites that had been identified as possible off-targeting sites.

“I think what is terrifically exciting about this is that it’s a very validated biology, but a very new approach,” says Andrew Bellinger, MD, PhD, chief scientific officer at Verve Therapeutics. “The excitement is the potential of this approach, [which can make] single base pair changes in one gene at one spot in your genome, [and which] can be so effective at turning off the gene so completely and so durably.”

Verve Therapeutics is focusing on patients who have genetic diseases like familial hypercholesteremia, and who are at high risk of atherosclerotic cardiovascular disease (ASCVD). The company also plans to eventually expand the treatment to anyone who has or is at risk for ASCVD.

“We can do this in very high-risk patients, and that’s the plan for the next few years,” Bellinger relates. “Ultimately, we think this approach of lipid nanoparticles delivering adenine base editors is one that’s very generalizable for larger patient populations.”

Exploring the new frontier with prime editing

Although base editors allow for a lot more specificity, they come with their own limitations. The main limitation is that base editors can do nothing but introduce transition point mutations by swapping one base pair for another. Base editors do not make insertions or deletions. However, an even newer technology called prime editing, again developed by Liu, can do much more.

Like base editors, prime editors can “nick” DNA (that is, cut just one of the two DNA strands) and bring about base-to-base conversions. In addition, prime editors can make small insertions and deletions. With these capabilities, prime editors could allow for the precise treatment of even more diseases caused by genetic mutations, about 90% of those listed in ClinVar.

In 2019, Liu co-founded Prime Medicine to develop prime editing as a search-and-replace technology. In a study from Liu’s group published the same year (Anzalone et al. Nature 2019; 576: 149–157), search-and-replace prime editing was described as the use of “a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase” that is programmed with “a prime editing guide RNA (pegRNA) that both specifies the target site and encodes the desired edit.”

In the study, over 175 edits were made across four different human cell lines, including insertions, deletions, and point mutations, with varying degrees of success. Overall, the researchers found that prime editing causes much less off-target editing than Cas9 and has strengths and weaknesses that complement those seen with base editing.

In one demonstration of prime editing’s abilities, a mutation that causes sickle-cell disease could be corrected with a gene editing efficiency of 44%, and an occurrence of indels, or unintended insertions and deletions, of about 5%.

“Prime editing makes the correct edit of a gene mutation at the exact target site and exhibits minimal off-target activity,” says Jennifer Gori, PhD, vice president of research at Prime Medicine. “Prime editing also does not cause double-strand breaks in chromosomal DNA or affect cell viability, which we believe may contribute to better patient outcomes, fewer side effects, and overall improved safety.”

The company, which was launched in July 2021 with $315 million in financing, will be partnering with another one of Liu’s companies, Beam Therapeutics, to develop multiple drug discovery programs.

“We are working on a variety of ‘delivery methods’ across multiple modalities, including methods such as nonviral nanoparticles and viral vectors,” Gori notes. “We look forward to further developing prime editing technology and progressing our preclinical programs toward the clinic. We are focused on making a difference to patients, and our hope is that our programs may cure or halt the progression of genetic diseases.”

Night Watchman:

NO ‘MICROCHIP’ NEEDED. Implantable Quantum Dot Microneedle Vaccine INFRARED Delivery System:

MIT researchers have now developed a novel way to record a patient’s vaccination history: storing the data in a pattern of dye, invisible to the naked eye, that is delivered under the skin at the same time as the vaccine. This technology could enable the rapid and anonymous detection of patient vaccination history. The researchers showed that their new dye, which consists of nanocrystals called quantum dots, can remain for at least five (5) years under the skin, where it emits [near-infrared light] that can be detected by a specially equipped smartphone. To create an “on-patient,” decentralized medical record, the researchers developed a new type of copper-based quantum dots, which emit light in the [near-infrared spectrum]. The dots are only about 4 nanometers in diameter, but they are encapsulated in biocompatible microparticles that form spheres about 20 microns in diameter. This encapsulation allows the dye to remain in place, under the skin, after being injected. The researchers designed their dye to be delivered by a microneedle ‘patch’ rather than a traditional syringe and needle. Such patches are now being developed to deliver vaccines for measles, rubella, and other diseases, and the researchers showed that their dye could be easily incorporated into these patches. The microneedles used in this study are made from a mixture of dissolvable sugar and a polymer called PVA, as well as the quantum-dot dye and the vaccine. When the patch is applied to the skin, the microneedles, which are 1.5 millimeters long, partially dissolve, releasing their payload within about two minutes.

By selectively loading microparticles into microneedles, the patches deliver a pattern in the skin that is invisible to the naked eye but can be scanned with a smartphone that has the [infrared] filter removed. The patch can be customized to imprint different patterns that correspond to the type of vaccine delivered. “It’s possible that this ‘invisible’ approach could create new possibilities for data storage, biosensing, and vaccine applications that could improve how medical care is provided, particularly in the developing world,” Langer says.

The quantum-dot patterns could be detected by smartphone cameras after up to five (5) years of simulated sun exposure. The researchers are also working on expanding the amount of data that can be encoded in a single pattern, allowing them to include information such as the date of vaccine administration and the lot number of the vaccine batch. “Storage, access, and control of medical records is an important topic with many possible approaches.” “This study presents a novel approach where the medical record is stored and controlled by the patient within the patient’s skin in a minimally invasive and elegant way.” The research was funded by the Bill and Melinda Gates Foundation and the Koch Institute Support (core) Grant from the National Cancer Institute.

8 References to ‘BE WATCHING or WATCHFUL.’ ‘8’ indicates that a new ‘era or epoch’ is arriving. Be it the rapture, the tribulation, the year of the Jews redemption or the year of the onset (2028) of the 1000 year millennial reign of Christ (2028-3028).

Matthew 24:42; Watch therefore: for ye know not what hour your Lord doth come.

Matthew 25:13; Watch therefore, for ye know neither the day nor the hour wherein the Son of man cometh.

Mark 13:35; Watch ye therefore: for ye know not when the master of the house cometh, at even, or at midnight, or at the cockcrowing, or in the morning.

Luke 21:36; Watch ye therefore, and pray always, that ye may be accounted worthy to escape all these things that shall come to pass, and to stand before the Son of man

Luke 12:37-39; Blessed are those servants, whom the lord when he cometh shall find watching: verily I say unto you, that he shall gird himself, and make them to sit down to meat, and will come forth and serve them. And if he shall come in the second watch, or come in the third watch, and find them so, blessed are those servants. And this know, that if the goodman of the house had known what hour the thief would come, he would have watched, and not have suffered his house to be broken through.

 ‘Increasing Like Labor Pains.’ ‘Fearful Sights.’ ‘Perilous Times.’ ‘Men’s hearts failing with fear.’ Great Convergence of Signs.’ REDEMPTION IMMINENT.

In His Service,

Night Watchman

Paul Rolland

Night Watchman Ministries

Make Your Decision for Christ NOW!!!!!!! Time is Up!!!!!!!

Jesus Christ’s Offer of Salvation:

The ABCs of Salvation through Jesus Christ (the Lamb)

A. Admit/Acknowledge/Accept that you are sinner. Ask God’s forgiveness and repent of your sins.

. . . “For all have sinned, and come short of the glory of God.” (Romans 3:23).

. . . “As it is written, There is none righteous, no, not one.” (Romans 3:10).

. . . “If we say that we have no sin, we deceive ourselves, and the truth is not in us.” (1 John 1:8).

B. Believe Jesus is Lord. Believe that Jesus Christ is who He claimed to be; that He was both fully God and fully man and that we are saved through His death, burial, and resurrection. Put your trust in Him as your only hope of salvation. Become a son or daughter of God by receiving Christ.

. . . “That whosoever believeth in him should not perish, but have eternal life. For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life. For God sent not his son into the world to condemn the world; but that the world through him might be saved. (John 3:15-17). For whosoever shall call upon the name of the Lord shall be saved.” (Romans 10:13).

C. Call upon His name, Confess with your heart and with your lips that Jesus is your Lord and Savior.

. . . “That if thou shalt confess with thy mouth the Lord Jesus, and shalt believe in thine heart that God hath raised him from the dead, thou shalt be saved. For with the heart man believeth unto righteousness; and with the mouth confession is made unto salvation.” (Romans 10:9-10).

. . . “If we say that we have no sin, we deceive ourselves, and the truth is not in us. If we confess our sins, he is faithful and just to forgive us our sins, and to cleanse us from all unrighteousness. If we say that we have not sinned, we make him a liar, and his word is not in us.” (John 1:8-10).

. . . “And he is the propitiation for our sins: and not for ours only, but also for the sins of the whole world. (John 2:2).

. . . “In this was manifested the love of god toward us, because that God sent his only begotten Son into the world, that we might live through him. And we have seen and do testify that the Father sent the Son to be the Saviour of the world. Whosoever shall confess that Jesus is the Son of God, God dwelleth in him, and he in God.” (1 John 4:9, 14-15).

. . . “But God commendeth his love toward us, in that, while we were yet sinners, Christ died for us. Much more then, being now justified by his blood, we shall be saved from wrath through him. For if, when we were enemies, we were reconciled to God by the death of his Son, much more, being reconciled, we shall be saved by his life.” (Romans 5:8-10).

. . . “For the wages of sin is death; but the gift of God is eternal life through Jesus Christ our Lord.” (Romans 6:23).

. . . “Jesus saith unto them, I am the way, the truth, and the life, no man cometh unto the Father, but by me.” (John 14:6).

. . . “For I am not ashamed of the gospel of Christ: for it is the power of God unto salvation to everyone that believeth.” (Romans 1:16).

. . . “Neither is there salvation in any other: for there is none other name under heaven given among men, whereby we must be saved.” (Acts: 4:12).

. . . “Who will have all men to be saved, and to come unto the knowledge of the truth for there is one God, and one mediator between God and men, the man Christ Jesus.” (1 Timothy 2:4-6).

. . . “For God did not appoint us to suffer wrath but to receive salvation through our Lord Jesus Christ.” (1 Thessalonians 5:9).

. . . “But as many as received him, to them gave the power to become the sons of God, even to them that believe on his name.” (John 1:12).

True Church / Bride of Christ Spared from God’s Wrath:

 Romans 5:8-10. “But God commendeth his love toward us, in that, while we were yet sinners, Christ died for us. Much more then, being now justified by his blood, we shall be saved from wrath through him. For if, when we were enemies, we were reconciled to God by the death of his Son, much more, being reconciled, we shall be saved by his life.”

Romans 12:19. Dearly beloved, avenge not yourselves, but rather give place unto wrath: for it is written, Vengeance is mine; I will repay, saith the Lord.

1 Thessalonians 1:10. And to wait for his Son from heaven, whom he raised from the dead, even Jesus, which delivered us from the wrath to come.

1 Thessalonians 5:9. For God hath not appointed us to wrath, but to obtain salvation by our Lord Jesus Christ,

Romans 8:35. Who shall separate us from the love of Christ? shall tribulation, or distress, or persecution, or famine, or nakedness, or peril, or sword?

Jeremiah 30:7. Alas! for that day is great, so that none is like it: it is even the time of Jacob’s trouble, but he shall be saved out of it.

Revelation 3:10 Because thou hast kept the word of my patience, I also will keep thee from the hour of temptation, which shall come upon all the world, to try them that dwell upon the earth.

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