The ‘coming’ mark will most likely involve a ‘subdermal’ application of the next wave of vaccinations, that will include a form of ‘digital data memory’ indicating date and time of vaccination. This ‘mark’ of vaccination will involve a sub-dermal outline or image representing a ‘branding’ that can be scanned and revealed by ultra-violet lite at a retail checkout point scanner. Just as many people have a physical vaccination ‘mark’ on their arm from getting shots in the 1960s, the coming vaccination mark will be subtle and reside ‘inside’ the human body affecting and indicating a genomic change in the recipient. This is an abomination viewed by God and causes the recipient to lose ‘Imager Status Before God’ and results in eternal damnation. The vaccination ‘mark’ has to be easily accessible by scanners and retail checkout points, hence the recipient will have the option of receiving the vaccination ‘patch’ either on their hand or forehead for ‘scannability.’
New CRISPR-based test for COVID-19 uses a smartphone camera
by Gladstone Institutes DECEMBER 5, 2020
Imagine swabbing your nostrils, putting the swab in a device, and getting a read-out on your phone in 15 to 30 minutes that tells you if you are infected with the COVID-19 virus. This has been the vision for a team of scientists at Gladstone Institutes, University of California, Berkeley (UC Berkeley), and University of California, San Francisco (UCSF). And now, they report a scientific breakthrough that brings them closer to making this vision a reality.
One of the major hurdles to combating the COVID-19 pandemic and fully reopening communities across the country is the availability of mass rapid testing. Knowing who is infected would provide valuable insights about the potential spread and threat of the virus for policymakers and citizens alike.
Yet, people must often wait several days for their results, or even longer when there is a backlog in processing lab tests. And, the situation is worsened by the fact that most infected people have mild or no symptoms, yet still carry and spread the virus.
In a new study published in the scientific journal Cell, the team from Gladstone, UC Berkeley, and UCSF has outlined the technology for a CRISPR-based test for COVID-19 that uses a smartphone camera to provide accurate results in under 30 minutes.
“It has been an urgent task for the scientific community to not only increase testing, but also to provide new testing options,” says Melanie Ott, MD, Ph.D., director of the Gladstone Institute of Virology and one of the leaders of the study. “The assay we developed could provide rapid, low-cost testing to help control the spread of COVID-19.”
The technique was designed in collaboration with UC Berkeley bioengineer Daniel Fletcher, Ph.D., as well as Jennifer Doudna, Ph.D., who is a senior investigator at Gladstone, a professor at UC Berkeley, president of the Innovative Genomics Institute, and an investigator of the Howard Hughes Medical Institute. Doudna recently won the 2020 Nobel Prize in Chemistry for co-discovering CRISPR-Cas genome editing, the technology that underlies this work.
Not only can their new diagnostic test generate a positive or negative result, it also measures the viral load (or the concentration of SARS-CoV-2, the virus that causes COVID-19) in a given sample.
“When coupled with repeated testing, measuring viral load could help determine whether an infection is increasing or decreasing,” says Fletcher, who is also a Chan Zuckerberg Biohub Investigator. “Monitoring the course of a patient’s infection could help health care professionals estimate the stage of infection and predict, in real time, how long is likely needed for recovery.”
Current COVID-19 tests use a method called quantitative PCR—the gold standard of testing. However, one of the issues with using this technique to test for SARS-CoV-2 is that it requires DNA. Coronavirus is an RNA virus, which means that to use the PCR approach, the viral RNA must first be converted to DNA. In addition, this technique relies on a two-step chemical reaction, including an amplification step to provide enough of the DNA to make it detectable. So, current tests typically need trained users, specialized reagents, and cumbersome lab equipment, which severely limits where testing can occur and causes delays in receiving results.
As an alternative to PCR, scientists are developing testing strategies based on the gene-editing technology CRISPR, which excels at specifically identifying genetic material.
All CRISPR diagnostics to date have required that the viral RNA be converted to DNA and amplified before it can be detected, adding time and complexity. In contrast, the novel approach described in this recent study skips all the conversion and amplification steps, using CRISPR to directly detect the viral RNA.
“One reason we’re excited about CRISPR-based diagnostics is the potential for quick, accurate results at the point of need,” says Doudna. “This is especially helpful in places with limited access to testing, or when frequent, rapid testing is needed. It could eliminate a lot of the bottlenecks we’ve seen with COVID-19.”
Parinaz Fozouni, a UCSF graduate student working in Ott’s lab at Gladstone, had been working on an RNA detection system for HIV for the past few years. But in January 2020, when it became clear that the coronavirus was becoming a bigger issue globally and that testing was a potential pitfall, she and her colleagues decided to shift their focus to COVID-19.
“We knew the assay we were developing would be a logical fit to help the crisis by allowing rapid testing with minimal resources,” says Fozouni, who is co-first author of the paper, along with Sungmin Son and María Díaz de León Derby from Fletcher’s team at UC Berkeley. “Instead of the well-known CRISPR protein called Cas9, which recognizes and cleaves DNA, we used Cas13, which cleaves RNA.”
In the new test, the Cas13 protein is combined with a reporter molecule that becomes fluorescent when cut, and then mixed with a patient sample from a nasal swab. The sample is placed in a device that attaches to a smartphone. If the sample contains RNA from SARS-CoV-2, Cas13 will be activated and will cut the reporter molecule, causing the emission of a fluorescent signal. Then, the smartphone camera, essentially converted into a microscope, can detect the fluorescence and report that a swab tested positive for the virus.
“What really makes this test unique is that it uses a one-step reaction to directly test the viral RNA, as opposed to the two-step process in traditional PCR tests,” says Ott, who is also a professor in the Department of Medicine at UCSF. “The simpler chemistry, paired with the smartphone camera, cuts down detection time and doesn’t require complex lab equipment. It also allows the test to yield quantitative measurements rather than simply a positive or negative result.”
The researchers also say that their assay could be adapted to a variety of mobile phones, making the technology easily accessible.
“We chose to use mobile phones as the basis for our detection device since they have intuitive user interfaces and highly sensitive cameras that we can use to detect fluorescence,” explains Fletcher. “Mobile phones are also mass-produced and cost-effective, demonstrating that specialized lab instruments aren’t necessary for this assay.”
Accurate and Quick Results to Limit the Pandemic
When the scientists tested their device using patient samples, they confirmed that it could provide a very fast turnaround time of results for samples with clinically relevant viral loads. In fact, the device accurately detected a set of positive samples in under 5 minutes. For samples with a low viral load, the device required up to 30 minutes to distinguish it from a negative test.
“Recent models of SARS-CoV-2 suggest that frequent testing with a fast turnaround time is what we need to overcome the current pandemic,” says Ott. “We hope that with increased testing, we can avoid lockdowns and protect the most vulnerable populations.”
Not only does the new CRISPR-based test offer a promising option for rapid testing, but by using a smartphone and avoiding the need for bulky lab equipment, it has the potential to become portable and eventually be made available for point-of-care or even at-home use. And, it could also be expanded to diagnose other respiratory viruses beyond SARS-CoV-2.
In addition, the high sensitivity of smartphone cameras, together with their connectivity, GPS, and data-processing capabilities, have made them attractive tools for diagnosing disease in low-resource regions.
“We hope to develop our test into a device that could instantly upload results into cloud-based systems while maintaining patient privacy, which would be important for contact tracing and epidemiologic studies,” Ott says. “This type of smartphone-based diagnostic test could play a crucial role in controlling the current and future pandemics.”
Explore further
For quick COVID-19 testing, iSCAN can be adopted for airports and public transportation
Night Watchman Note; ‘THE GRAND DADDY SIGN’. ‘THAT WICKED’. That is what ‘watchmen or women’ do. Jesus made clear that when He actually does come for His disciples, His wise and faithful servants would be aware of the time because they would be watching. They would know the season of the times by WATCHING those things ‘coming to pass or occurring’ as Jesus said they would. The ‘BENCHMARK SIGN(s)’ always point towards Israel / Jacob. God’s prophetic timepiece. The FALSE PEACE COVENANT confirmed by the Prince/Beast among MENA (the many) is the ‘key sign’ of the times or the season of the times. Yes, we are in that period. The Prince/Beast will likely confirm and enhance the current ‘normalization’ agreement with Jacob to be a ‘peace’ agreement, to be extended to 7 years from the current 5 years, to include some agreement permitting the rebuilding of the third temple in exchange for ‘some portion of Jerusalem.’ The current ‘investment’ period for the Palestinians stands at five (5) years. This will likely occur AFTER the Bride, Disciples or True Church is removed from the earth via the Rapture / Harpazo / Redemption / ‘Twinkling’.
Confirmation for Seven (7) Years
Daniel 9:26-27 And after threescore and two weeks shall Messiah be cut off, but not for himself: and the people of the prince that shall come shall destroy the city and the sanctuary; and the end thereof shall be with a flood, and unto the end of the war desolations are determined. And he shall confirm the covenant with many for one week: and in the midst of the week he shall cause the sacrifice and the oblation to cease, and for the overspreading of abominations he shall make it desolate, even until the consummation, and that determined shall be poured upon the desolate.
‘Current Plan’ (Satan’s Plan = Five (5) years financial investment period for 180 (6+6+6) ‘infrastructure projects’ costing $50 (5) Billion to cut poverty by 50% (5)).
U.S. President Donald Trump’s “Peace to Prosperity” plan, which he unveiled at the White House on Jan. 28, 2020, calls for Israel to retain 30 percent of the West Bank (territory that the parties to the conflict had already agreed would remain under Israeli control, in accordance with the 1995 Oslo Accords), with the remaining 70 percent reserved for an eventual Palestinian state. The plan states that there would be a freeze on Israeli settlement-building in that area for four years—and land swaps to provide the Palestinian state with land comparable in size to the territory of pre-1967 West Bank and Gaza, and a capital in eastern Jerusalem.
It includes a $50 billion investment fund for 179 infrastructure and business projects in that state, designed to create more than one million jobs, cut the poverty rate by 50 percent and reduce unemployment from 31 percent to a single digit.
US President Donald Trump has predicted that Saudi Arabia will be among up to 10 further countries preparing to ‘normalize’ their relations with Israel.
The meaning was obvious: The country [Saudi Arabia] is tired of waiting for Palestinians to make peace and is preparing to move on. The message to foreign capitals, perhaps to Washington in particular, is equally clear: The time is coming when a deal may be done.
Prophetic Discernment: Two ‘Scenarios’. I am not ‘dogmatic’ about either one. Other scenarios could come into play. THE POINT … is how close we are!
Trump may still yet get the Beast to agree for ‘MENA’ the ‘CURRENT’ plan, SHORTLY, in the few remaining days left in the ‘Last Trump’ or ‘Last Trumpence’ Presidency. Saudis may be ‘nervous’ about the Biden election and want to get the ‘deal done and finished’ while Trump is in the twilight of his presidency.
The Rapture
The following would take place AFTER the Rapture: Biden/Harris may work to ‘ENHANCE’ the plan by the literal splitting of Jerusalem along the 1967 lines. An ‘exchange’ of sovereignty to get a temple for the Jews and to give the Beast custodianship of al-Aqsa … that which he covets. The plan will be ‘ENHANCED’ (investment wise for the Palestinians) for Seven (7) years. Perhaps the investment amount will also be increased.
Or
Rapture
Then the following: Beast ‘ENHANCES’ the plan under Biden, by splitting Jerusalem along 1967 lines. In a ‘Give up Sovereignty, Get a Temple’ scenario. The plan finally gets ‘CONFIRMED’ or approved shortly, under Biden/Harris (Deceiver-in-Chief and Jezebel-in-Chief).
Either way, the Rapture / Harpazo / Redemption / ‘Twinkling’ is imminent.
Prophecy has a ‘window.’ That window is closing shortly and quickly. As is the door in the ‘Age of Grace’ for the world.
Pay ‘Eagle-Eye’ attention to the world geo-political news feeds to see if any type of ‘confirmation’ is forthcoming or made by the Prince / Beast/ 8th King / Son of Perdition / Future Antichrist / Man of Lawlessness. Also MbS, Man-by-Satan and the Confirmer and he that causeth the Abomination of Desolation in April 2024. Trump may surprise the world and suddenly proclaim that a deal has been reach or agreed upon by Saudi Arabia and at least nine (3×3) other Arab/Muslim countries from MENA. If nothing happens over the next 10 days, it is possible the entire covenant could be ‘strengthened and confirmed’ AFTER the rapture. This is a very real possibility. Be warned and hopeful.
Seven (7) References to ‘BE WATCHING or WATCHFUL.’
Matthew 24:42; Watch therefore: for ye know not what hour your Lord doth come.
Matthew 25:13; Watch therefore, for ye know neither the day nor the hour wherein the Son of man cometh.
Mark 13:35; Watch ye therefore: for ye know not when the master of the house cometh, at even, or at midnight, or at the cockcrowing, or in the morning.
Luke 21:36; Watch ye therefore, and pray always, that ye may be accounted worthy to escape all these things that shall come to pass, and to stand before the Son of man
Luke 12:37-39; Blessed are those servants, whom the lord when he cometh shall find watching: verily I say unto you, that he shall gird himself, and make them to sit down to meat, and will come forth and serve them. And if he shall come in the second watch, or come in the third watch, and find them so, blessed are those servants. And this know, that if the goodman of the house had known what hour the thief would come, he would have watched, and not have suffered his house to be broken through.
1 Thessalonians 5:2-4; For yourselves know perfectly that the day of the Lord so cometh as a thief in the night. For when they shall say, Peace and safety; then sudden destruction cometh upon them, as travail upon a woman with child; and they shall not escape. But ye, brethren, are not in darkness, that that day should overtake you as a thief. (Be Watching).
John 13:19 Now I tell you before it come, that, when it is come to pass, ye may believe that I am he.
John 14:29 And now I have told you before it come to pass, that, when it is come to pass, ye might believe.
Luke 21:31 So likewise ye, when ye see these things come to pass, know ye that the kingdom of God is nigh at hand.
Mark 13:29 So ye in like manner, when ye shall see these things come to pass, know that it is nigh, even at the doors.
Luke 21:28 And when these things begin to come to pass, then look up, and lift up your heads; for your redemption draweth nigh.
Revelation 1:1 The Revelation of Jesus Christ, which God gave unto him, to shew unto his servants things which must shortly come to pass; and he sent and signified it by his angel unto his servant John:
‘Increasing Like Labor Pains.’ ‘Fearful Sights.’ ‘Perilous Times.’ ‘Men’s hearts failing with fear.’ Great Convergence of Signs.’ REDEMPTION IMMINENT.
In His Service,
Night Watchman
Paul Rolland
Night Watchman Ministries
Make Your Decision for Christ NOW!!!!!!! Time is Up!!!!!!!
Jesus Christ’s Offer of Salvation:
The ABCs of Salvation through Jesus Christ (the Lamb)
A. Admit/Acknowledge/Accept that you are sinner. Ask God’s forgiveness and repent of your sins.
. . . “For all have sinned, and come short of the glory of God.” (Romans 3:23).
. . . “As it is written, There is none righteous, no, not one.” (Romans 3:10).
. . . “If we say that we have no sin, we deceive ourselves, and the truth is not in us.” (1 John 1:8).
B. Believe Jesus is Lord. Believe that Jesus Christ is who He claimed to be; that He was both fully God and fully man and that we are saved through His death, burial, and resurrection. Put your trust in Him as your only hope of salvation. Become a son or daughter of God by receiving Christ.
. . . “That whosoever believeth in him should not perish, but have eternal life. For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life. For God sent not his son into the world to condemn the world; but that the world through him might be saved. (John 3:15-17). For whosoever shall call upon the name of the Lord shall be saved.” (Romans 10:13).
C. Call upon His name, Confess with your heart and with your lips that Jesus is your Lord and Savior.
. . . “That if thou shalt confess with thy mouth the Lord Jesus, and shalt believe in thine heart that God hath raised him from the dead, thou shalt be saved. For with the heart man believeth unto righteousness; and with the mouth confession is made unto salvation.” (Romans 10:9-10).
. . . “If we say that we have no sin, we deceive ourselves, and the truth is not in us. If we confess our sins, he is faithful and just to forgive us our sins, and to cleanse us from all unrighteousness. If we say that we have not sinned, we make him a liar, and his word is not in us.” (John 1:8-10).
. . . “And he is the propitiation for our sins: and not for ours only, but also for the sins of the whole world. (John 2:2).
. . . “In this was manifested the love of god toward us, because that God sent his only begotten Son into the world, that we might live through him. And we have seen and do testify that the Father sent the Son to be the Saviour of the world. Whosoever shall confess that Jesus is the Son of God, God dwelleth in him, and he in God.” (1 John 4:9, 14-15).
. . . “But God commendeth his love toward us, in that, while we were yet sinners, Christ died for us. Much more then, being now justified by his blood, we shall be saved from wrath through him. For if, when we were enemies, we were reconciled to God by the death of his Son, much more, being reconciled, we shall be saved by his life.” (Romans 5:8-10).
. . . “For the wages of sin is death; but the gift of God is eternal life through Jesus Christ our Lord.” (Romans 6:23).
. . . “Jesus saith unto them, I am the way, the truth, and the life, no man cometh unto the Father, but by me.” (John 14:6).
. . . “For I am not ashamed of the gospel of Christ: for it is the power of God unto salvation to everyone that believeth.” (Romans 1:16).
. . . “Neither is there salvation in any other: for there is none other name under heaven given among men, whereby we must be saved.” (Acts: 4:12).
. . . “Who will have all men to be saved, and to come unto the knowledge of the truth for there is one God, and one mediator between God and men, the man Christ Jesus.” (1 Timothy 2:4-6).
. . . “For God did not appoint us to suffer wrath but to receive salvation through our Lord Jesus Christ.” (1 Thessalonians 5:9).
. . . “But as many as received him, to them gave the power to become the sons of God, even to them that believe on his name.” (John 1:12).
True Church / Bride of Christ Spared from God’s Wrath:
Romans 5:8-10. “But God commendeth his love toward us, in that, while we were yet sinners, Christ died for us. Much more then, being now justified by his blood, we shall be saved from wrath through him. For if, when we were enemies, we were reconciled to God by the death of his Son, much more, being reconciled, we shall be saved by his life.”
Romans 12:19. Dearly beloved, avenge not yourselves, but rather give place unto wrath: for it is written, Vengeance is mine; I will repay, saith the Lord.
1 Thessalonians 1:10. And to wait for his Son from heaven, whom he raised from the dead, even Jesus, which delivered us from the wrath to come.
1 Thessalonians 5:9. For God hath not appointed us to wrath, but to obtain salvation by our Lord Jesus Christ,
Romans 8:35. Who shall separate us from the love of Christ? shall tribulation, or distress, or persecution, or famine, or nakedness, or peril, or sword?
Jeremiah 30:7. Alas! for that day is great, so that none is like it: it is even the time of Jacob’s trouble, but he shall be saved out of it.
Revelation 3:10 Because thou hast kept the word of my patience, I also will keep thee from the hour of temptation, which shall come upon all the world, to try them that dwell upon the earth.